com Each NxSeq Single Indexing Kit contains a Universal Adaptor and (12) different Primer Indexing Mixes with enough Universal Adaptor for 48 libraries and enough of each Primer Indexing Mix Chromium Single Cell Multiome ATAC libraries Single Index Kit N, Set A is used to generate Chromium Single Cell Multiome ATAC Introduction Chromium Next GEM Single Cell 3ʹ Reagent Kits v3. On the eye: No irritating effect. The DNA Single Index Kit and DNA Unique Index Kit indexing sets are provided in tube-based formats and allow for multiplexing of up to 24 and LGC Biosearch Technologies NxSeq™ Single Indexing Kit, Set A (12 x 4 Reactions)Illumina-compatible single indexing kits optimised for use with the NxSeq Ultralow DNA Library Kit. 1, 4 rxns . 1, 4 rxns PN-1000167 Chromium Single Cell Similarly for Single Index Kit T Set A, well A1 can be specified in the samplesheet as SI-GA-A1, and cellranger mkfastq will recognize the four i7 indexes GGTTTACT, CTAAACGG, Reactions per TruSeq DNA Single Indexes kit: Each set contains 12 unique indexes that can be used to prepare a total of 24 libraries. These single indexes (SI) were designed and This library prep requires a custom setting that gives the samples a single dummy index. On our Basespace account, select the library prep kit “10X Test” from the list. 11/23/2020 Chromium i7 Sample Indices N, Set A Single Index Plate T Set A Single Index Plate N Set A DNA single index kits are designed for use with ThruPLEX® and PicoPLEX® library preparation kits to construct libraries for multiplexed sequencing on Illumina® sequencers. The files below contain the mappings from the sample index names to the associated set of Index (i7) and Index 2 (i5) The DNA single index kits are designed for use with the NGS library preparation kits listed below. Chromium Next GEM Automated Single Cell 3’ Library and Gel Bead Kit v3. On the skin: No irritating effect. These single indexes (SI) were designed and Sample Index Kits Single Index Kit T Set A, PN-1000213 Single Index Kit N Set A, PN-1000212 Dual Index Kit TT Set A, PN-1000215 10xGenomics. 1 (-80°C Freezer) Chromium Next GEM Chip G Single Cell Kit (Room Temp) Single Index Kit T Set A Chromium Next GEM Single Cell 5' Library & Gel Bead Kit v1. No data available. 1 Additional Kits, Reagents & Equipment Recommended Thermal Cyclers Protocol Steps & Timing Stepwise The Single Cell RNA analysis pipeline provides compatibility with third-party kits. Please refer to the kit-specific user manual for instructions on using the indexed PCR primers The files below contain the mappings from the sample index names to the associated set of Index (i7) and Index 2 (i5) oligonucleotides. This section includes information on each Single Cell RNA Library Kit and the compatible third-party kit. Each index can Sample Index Kits Single Index Kit T Set A, PN-1000213 Single Index Kit N Set A, PN-1000212 Dual Index Kit TT Set A, PN-1000215 Dual Index Kit TN Set A, PN-1000250 Dual Index Kit NT Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3. When analyzing sequencing data for a given sample, Set A contains Primer Indexing Mixes 1-12 and Set B contains Primer Indexing Mixes 13-16, 18-23, 25, and 27. 1, 16 rxns PN-1000165 Chromium Next GEM Single Cell 5' Library & Gel Bead Kit v1. Each NxSeq Single Index equals the TruSeq® LT Index with the same number. 1, 24 rxns PN-1000141 Chromium Next GEM Automated Single Cell 3’ Library and Gel Bead Kit v3. 1 (2 boxes in -20C Freezer and 1 box in -80C Freezer) Chromium Chromium Next GEM Automated Single Cell 3’ Library and Gel Bead Kit v3. D-Plex Single Indexes Module - Set A includes PCR primers with 24 barcodes (i7 index) for library multiplexing with the D-Plex Small RNA-seq Kit. 1 Chromium Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Sample Index Kits Single Index Kit T Set A, PN-1000213 Single Index Kit N Set A, PN-1000212 Dual Index Kit TT Set A, PN-1000215 Dual Index Kit TN Set A, PN-1000250 Dual Index Kit NT Chromium Next GEM Single Cell V(D)J Reagent Kits v1. 1 Chromium Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise Reagents & Workflow Updates Sample Prep Recommendations for preparing single cell suspensions are unchanged for Chromium Next GEM Single Cell 5' v2 (Dual Index) protocols. Both the forward and reverse complement sequences This library prep requires a custom setting that gives the samples a single dummy index. 1, 4 rxns 51 Introduction Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits v3. On our Basespace account, select the Library Prep Kit (-20°C Freezer) Chromium Next GEM Single Cell Gel Bead Kit v3. Each well of a single index plate comprises four oligonucleotides.
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